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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:50855*  Cite 

Wild type Saccharomyces cerevisiae cells (S228C background) were treated with 1 mg/ml of the proline analogue azetidine-2-carboxylic acid (AZC) for 30 and 90 minutes. Then, samples were high pressure frozen and visualized through transmission electron microscopy (TEM). The images show the nucleus of the cells and the black arrow indicates nuclear envelope budding events.

Technical Details

Treated cells were cultured in YPD medium and high pressure frozen in a Wohlwend Compact 3 machine, followed by a short freeze substitution protocol in 2% Uranyl acetate and embedded in HM20 resin. Sections of 70 nm thickness were then contrast stained with 2% Uranyl acetate and Reynold's lead citrate. Pictures were aquired in a Tecnai T12 TEM with a Ceta CMOS 16M camera.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae (baker's yeast, budding yeast)
Cell Type
Mixed population of S. cerevisiae cells
Cellular Component
Biological Context
Biological Process
Nuclear envelope budding
Johanna Höög
Dimitra Panagaki
Jacob Croft
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Mode
transmission electron microscopy (TEM)
Visualization Methods
Stain with Uranyl acetate and Lead citrate