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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:50725*  Cite 
Description

FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. Postnatal day 4 mouse Inner Ear Organ of Corti labeled with anti-PKHD1L1 antibody (10 nm gold beads). The dataset represents the apical end of the Outer Hair Cell with the stereocilium bundle. Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.

Technical Details

Images were acquired with FEI Helios FIB-SEM, using backscatter detector from an epoxy resin embedded inner ear Organ of Corti sample.

7.1_Test_Cell_Stereocilia_Segmentation is a multipage TIFF file illustrating stereocilia segmentation results of Cell#3. Same as ‘Towers.am’ file used for MATLAB volume analysis of this cell, but converted to TIFF to allow for online preview.

This file is a part of Dataset 7, comprised of files used as a technical validation dataset. It is an artificial dataset with manually placed gold beads, used to access the accuracy of the MATLAB script. The choice of gold bead placement location was unique for each row of stereocilia to control for potential errors of stereocilia placement within the rows during MATLAB analysis. No beads were placed on the surface of the kinocilium.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
Inner Ear Outer Hair Cell
Cellular Component
Hair Cell stereocilia
Cuticular plate
Mitochondria
Polycystic Kidney and Hepatic Disease 1-Like 1 (PKHD1L1) labeling (gold beads)
Biological Context
Biological Process
Native auditory Hair Cells at postnatal day 4
Attribution
Names
Maryna V. Ivanchenko
Marcelo Cicconet
Hoor Al Jandal
Xudong Wu
David P. Corey (http://corey.med.harvard.edu/)
Artur A. Indzhykulian (https://ailab.hms.harvard.edu/)
Link
Scientific Data article
Nature Communications article
OTHER
Massachusetts Eye and Ear; Harvard Medical School
7.1_Test_Cell_Stereocilia_Segmentation
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL50725
Archival Resource Key (ARK)
ark:/b7295/w9cil50725
Grouping This image is part of a group.
Imaging
Image Mode
Serial Electron Microscopy
Focused Ion Beam Scanning Electron Microscopy (FIB-SEM)
Visualization Methods
Heavy metal stain (Osmium Tetroxide, Potassium Ferrocyanide, Tannic Acid)
Immunogold labeling with 10 nm gold beads