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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:49759*  Cite 

High-resolution montage of a thin section of Healthy human skin, including part of the dermis and all epidermal layers. This image is a montage of 32 images and a series of 10 datasets as published:

Technical Details

Two mm skin were fixed in 2% glutaraldehyde in 0.1 M sodium cacodylate buffer pH 7.4 washed in 0.1 M sodium cacodylate buffer and postfixed in 1% osmiumtetroxide and 1.5% potassiumferrocyanide. Samples were dehydrated, embedded in epon, and sectioned. Ultrathin sections (70 nm) were positioned on single slot holders A600 Nickel supported by Formvar and contrasted with 2% uranylacetate (methanol) followed by Reynolds lead citrate. Acquisition was performed using a Zeiss supra 55 EM (Oberkochen, Germany) with ATLAS software developed by Fibics (Ottawa, Ontario, Canada). Samples were recorded at 2.5 nm pixel size. Scans were stitched, and raw data sets were rendered as HTML files using ATLAS VE viewer software. data sets are orientated such that the epidermis/superficial layers are facing up.

Biological Sources
NCBI Organism Classification
Lesional skin
Biological Context
Biological Process
staphylococcal scalded skin syndrome
Ena Sokol
Duco Kramer
Gilles Diercks
Jeroen Kuipers
Marcel Jonkman
Hendri Pas
Ben Giepmans
JID article
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
electron density
Sample Preparation
glutaraldehyde fixed tissue
ultrathin section
Spatial Axis Image Size Pixel Size
X 138px 55.296µm
Y 54px 21.504µm
Z 54px 21.504µm