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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:49701*  Cite 
Description

High-resolution montage of a thin section of Healthy human skin, including part of the dermis and all epidermal layers. This image is a montage of 32 images and a series of 10 datasets as published: http://www.jidonline.org/article/S0022-202X(15)37322-X/fulltext http://www.nanotomy.org/OA/Sokol2015JID/index.html

Technical Details

Two mm skin were fixed in 2% glutaraldehyde in 0.1 M sodium cacodylate buffer pH 7.4 washed in 0.1 M sodium cacodylate buffer and postfixed in 1% osmiumtetroxide and 1.5% potassiumferrocyanide. Samples were dehydrated, embedded in epon, and sectioned. Ultrathin sections (70 nm) were positioned on single slot holders A600 Nickel supported by Formvar and contrasted with 2% uranylacetate (methanol) followed by Reynolds lead citrate. Acquisition was performed using a Zeiss supra 55 EM (Oberkochen, Germany) with ATLAS software developed by Fibics (Ottawa, Ontario, Canada). Samples were recorded at 2.5 nm pixel size. Scans were stitched, and raw data sets were rendered as HTML files using ATLAS VE viewer software. data sets are orientated such that the epidermis/superficial layers are facing up.

Biological Sources
NCBI Organism Classification
Normal human skin
Biological Context
Biological Process
Blister diseases
Attribution
Names
Ena Sokol
Duco Kramer
Gilles Diercks
Jeroen Kuipers
Marcel Jonkman
Hendri Pas
Ben Giepmans
Link
nanotomy.org
JID article
Zeiss viewer
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL49701
Archival Resource Key (ARK)
ark:/b7295/w9cil49701
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Visualization Methods
electron density
Sample Preparation
Methods
glutaraldehyde fixed tissue
Methods
ultrathin section
Dimensions
Spatial Axis Image Size Pixel Size
X 325px 130.048µm
Y 182px 72.704µm
Z 182px 72.704µm