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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:48306*  Cite 

Images are acquired from fixed and living heart tissue using fiber-optics and laser-scanning confocal microscopy, respectively. Three sets of images denoted as CCM, FCMtopical, FCMcarrier were stored for subsequent analyses.

Technical Details

The imaging protocol was based on topical application of 3 or 10 kDa dextran conjugated to Alexa Fluor 488 (Invitrogen, Carlsbad, CA, 125 μg/mL) via pipette to the tissue regions prior to imaging. Immediately following, images were acquired using the fiber-optics confocal microscopy system equipped with a custom fiber-optics microprobe (UltraMiniO; Mauna Kea Technologies, Paris, France). Using this imaging protocol, we acquired two-dimensional images sequences of the tissue regions at a lateral resolution (xy dimensions), optical sectioning (z), field of view (xy), frame rate, and z-scan range of 1.8 μm, 10 μm, 169 by 120 μm, 12 Hz and 50 μm, respectively. Example images from image sequences were stored as FCMtopical.

Biological Sources
NCBI Organism Classification
Sprague-Dawley rat
Cellular Component
nodal cells
Frank Roels
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Mode
confocal microscopy
Sample Preparation
chemically fixed tissue
Spatial Axis Image Size Pixel Size
X —— 169µm
Y —— 120µm
Z —— 50µm