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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:48304*  Cite 

Images are acquired from fixed and living heart tissue using fiber-optics and laser-scanning confocal microscopy, respectively. Three sets of images denoted as CCM, FCMtopical, FCMcarrier were stored for subsequent analyses.

Technical Details

Tissue regions were labeled with a dye carrier affixed to the tip of a fiber-optics microprobe (UltraMiniOWD30; Mauna Kea Technologies). Dye carriers were loaded for 15 min with sodium fluorescein [Fluorescite® (fluorescein injection, USP) 10%; Alcon, Fort Worth, TX, US; 1:1000] prior to imaging. With this imaging protocol, we acquired two-dimensional image sequences of the tissue regions at a lateral resolution, optical sectioning, field of view, frame rate, and z-scan range of 1.4 μm, 7 μm, 186 by 130 μm, 12 Hz and 26 μm, respectively. Example images from these image sequences were stored as FCMcarrier.

Biological Sources
NCBI Organism Classification
Sprague-Dawley rat
Cellular Component
nodal cells
Frank Roels
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Mode
confocal microscopy
Sample Preparation
chemically fixed tissue
Spatial Axis Image Size Pixel Size
X —— 186µm
Y —— 130µm
Z —— 26µm