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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:48106*  Cite 
Description

Rat cardiomyocytes were plated and stained for actin (in red), desmin (in green) to reveal the intermediate filament essential for the structural integrity and function of muscle, and DAPI (in blue) to label nuclei.

Technical Details

Plated rat cardiomyocytes were fixed and immunostained for actin (from Santa Cruz), desmin (from Sigma), and DAPI (from Life Technologies). Images were acquired with a 40X (Plan Fluor, NA 0.75) lens, SPOT RT Slider CCD camera and SPOT Basic image capture software. Image generated with the Image J color combine function.

Biological Sources
NCBI Organism Classification
Rattus norvegicus
Cell Type
fetal cardiomyocyte
Cellular Component
cytoskeleton
nucleus
Attribution
Names
Natalie Prigozhina
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL48106
Archival Resource Key (ARK)
ark:/b7295/w9cil48106
Imaging
Image Type
charge coupled device (CCD)
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
4',6-diamidino-2-phenylindole (DAPI)
primary antibody plus labeled secondary antibody
Data Qualifiers
processed data
Sample Preparation
Relation To Intact Cell
dispersed cells in vitro
Dimensions


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