Cryopreserved human mammary epithelial cells were revived and stained for cytokeratin 18 (in red) to reveal the keratin-containing intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue, E-cadherin (in green) to visualize the calcium-dependent cell-cell adhesion glycoprotein, and DAPI (in blue) to label nuclei.
Cryopreserved human mammary epithelial cells were fixed and stained for cytokeratin 18, E-cadherin (from BD Transduction), and DAPI (from Life Technologies). Images were acquired with a 40X (Plan Fluor, NA 0.75) lens, SPOT RT Slider CCD camera and SPOT Basic image capture software. Image generated with the Image J color combine function.
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