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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:48014*  Cite 

Single computed slice through a tomographic reconstruction of a selectively-stained spiny dendrite. This medium spiny neuron was contained in a thick section from the neostriatum of a dopamine transporter knock out mouse. The reconstructed image has been downsampled from the raw data image, which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Micer were C57BL/129SvJ. Individual cells in fixed tissue were injected with Lucifer yellow and stored in 4% paraformaldehyde at 4° C. Fluorescence was photoconverted using DAB and a confocal laser light source. After this process, tissue was washed thoroughly in PBS and processed for EM by osmication, dehydration, and embedding in epoxy resin. For detailed methods, see the protocol provided in the CCDB. The tomogram was generated using an Hitachi 3MeV UHVEM. Single tilt images spanned -70 to 70° in 2° increments.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
CNS neuron (sensu Vertebrata)
Cellular Component
dendritic spine
Diana Price
Aki Laakso
Michele Cyr
Maryann Martone
Naoko Yamada
Andrea Thor
Monica Berlanga
CCDB 4003
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
detection of electrons
illumination by electrons
Parameters Imaged
electron density
Visualization Methods
intracellular filling
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——