Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:47523^* Cite

Description

Visualization of neutrophil recruitment in capillaries proximal to S. aureus beads. (Figure 3 - A4 block)

Technical Details

Mice were anesthetized with ketamine/xylazine, and then subcutaneous tissue on a dorsal flap was exteriorized. GFP labelled S. aureus beads were inserted. Antibodies were injected into the vasculature to label neutrophils (red) and endothelial cells (blue)

Biological Sources

NCBI Organism Classification: Staphylococcus aureus
Cell Type: neutrophil; endothelial cell

Biological Context

Biological Process
Molecular Function: blocking of integrin α4

Attribution

Names: Mark Harding

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL47523
Archival Resource Key (ARK): ark:/b7295/w9cil47523

Imaging

Image Type: recorded image
Image Mode: spinning disk confocal microscopy
Processing History: Converted from volocity to .avi

Sample Preparation

Relation To Intact Cell: intact cells-in vivo imaging

Dimensions

Spatial Axis	Image Size	Pixel Size
X	——	512µm
Y	——	512µm