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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:4661*  Cite 

A high resolution micrograph of the locomotor cortex of Didinium. A longitudinal section reveals the basal bodies and the axonemes of cilia as they emerge from the cell cortex of Didinium. Bundles of microtubules extend from the basal bodies into the endoplasm through the fibrous layer that characterizes the cortex of Didinium. Tubular or flattened early endosomes with coated pits at their margins are present in the endoplasm beneath the fibrous layer. Mitochondrial cristae appear to be decorated by F1F0 ATP synthases. Cross sections of three bacteria are noted between the cilia. TEM taken on 5/9/69 by M. Sage with Philips 300 operating at 60kV. Neg. 14,800X. The raw film was scanned with a Nikon Coolscan 9000ED. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (

Biological Sources
NCBI Organism Classification
Didinium nasutum
Cell Type
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Carolina Biological Supply Company, NC, U.S.A.
Cellular Component
cell cortex
microtubule basal body
ciliary rootlet
Biological Context
Biological Process
ciliary cell motility
microtubule cytoskeleton organization
Molecular Function
cytoskeletal protein binding
M. Sage
Richard Allen
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Spatial Axis Image Size Pixel Size
X 5582px 1nm
Y 3792px 1nm