The Cell: An Image Library

Licensing

Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more

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CIL:4657^*

Description

Purified rabbit skeletal muscle myosin in 0.6M KCl was adsorbed onto mica flakes, quick frozen by contact with a liquid helium-cooled copper block in a Heuser-type cryopress (Heuser JE, J. Mol.Biol. 1983. 169:155-195). The sample was freeze-fractured and deep-etched in a Balzers 400 freeze fracture device, and rotary-replicated in Pt-C at 11 degrees. This image was recorded at 150,000x in a JEOL 100CX2 transmission electron microscope operating at 100 kev, on Kodak 4489 film. The image was digitized using a Nikon Coolscan 9000ED scanner at 20 micron pixel size.

Biological Sources

NCBI Organism Classification: Oryctolagus cuniculus; (European rabbit)
Cell Type: skeletal muscle cell
Cellular Component: cytoskeleton

Biological Context

Biological Process: skeletal muscle contraction
Molecular Function: ATPase activity, coupled to movement of substances

Attribution

Name: Wallace Ip

Grouping

This image is part of a group.

Imaging

Image Type: recorded image
Imaging Mode: transmission electron microscopy (TEM); wide-field detection; widefield illumination; illumination by electrons; detection of electrons
Parameters Imaged: electron density; elevation
Source of Contrast: differences in deposition of metal shadow
Visualization Methods: shadowing and plating
Processing History: unprocessed raw data

Sample Preparation

Methods: unfixed tissue; freeze_fracture/freeze_etch; whole mounted tissue
Relation To Intact Cell: isolated subcellular component

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4149px	0.000133nm
Y	2797px	0.000133μm

*CIL – Cell Image Library accession number. Please use this to reference an image.