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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:45752*  Cite 
Description

Plate 20589 - Phenotypic profiling attempts to summarize multiparametric, feature-based analysis of cellular phenotypes of each sample so that similarities between profiles reflect similarities between samples. This image set provides a basis for testing image-based profiling methods wrt. to their ability to distinguish the effects of small molecules. Images The images are of U2OS cells treated with each of 1600 known bioactive compounds and labeled with six labels that characterize seven organelles (the .cell-painting. assay). This pilot experiment consists of 20 plates. Each plate has 384 wells and each well has 9 fields of view for a total of 69,120 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale image file, so there are 345,600 image files in 16-bit TIFF format.

Technical Details

Gustafsdottir et al. (doi:10.1371/journal.pone.0080999) have developed a multiplex cytological profiling assay that "paints the cell" with as many fluorescent markers as possible without compromising our ability to extract rich, quantitative profiles in high throughput. The assay detects seven major cellular components. In a pilot screen of bioactive compounds, the assay detected a range of cellular phenotypes and it clustered compounds with similar annotated protein targets or chemical structure based on cytological profiles. The results demonstrate that the assay captures subtle patterns in the combination of morphological labels, thereby detecting the effects of chemical compounds even though their targets are not stained directly. This image-based assay provides an unbiased approach to characterize compound- and disease-associated cell states to support future probe discovery. Using the cell-painting assay, the Broad Institute has assembled a reference dataset of profiles for U2OS osteosarcoma cells treated with ~30,000 compounds. The compound collection includes DOS-derived compounds (20,000), as well as chemically diverse MLI compounds with biologically diverse performance identified through analysis of PubChem (10,000), and known bioactive compounds to serve as landmarks (2,500). The DOS compounds consist of structurally diverse and stereochemically rich compounds with structures distinct from the current MLSMR. The compound collection also includes 267 distinct compounds nominated by MLPCN Centers from projects for which the Centers would like to identify new chemical series with similar activities. The experiment consists of 413 microtiter plates. Each plate has 384 wells. Each well has 9 fields of view. Each field was imaged in five channels (detection wavelengths), and each channel is stored as a separate, grayscale 16-bit TIFF image file.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Line
U-2 OS
Cellular Component
cell
Biological Context
Biological Process
response to chemical stimulus
Attribution
Names
Sigrun Gustafsdottir
Vebjorn Ljosa
Katherine Sokolnicki
Joshua Bittker
Nicole Bodycombe
Mark Bray
Vlado Dancik
Tom Hasaka
Suk Hon
Melissa Kemp
Kejie Li
Deepika Walpita
Mathias Wawer
Todd Golub
Stuart Schreiber
Paul Clemons
Anne Carpenter
Alykhan Shamji
Link
Broad Institute
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL45752
Archival Resource Key (ARK)
ark:/b7295/w9cil45752
Compounds
10-hydroxycamptothecin
1,25-DIHYDROXYVITAMIN D3
13-CIS RETINOIC ACID
17-Allylamino-geldanamycin
17-PHENYL-TRINOR-PGE2
24,25-DIHYDROXYVITAMIN D3
2-ARACHIDONOYLGLYCEROL
2-CHLORO-11-(4-METHYLPIPERAZINO)DIBENZ[B,F]OXEPIN
3,4-dichloroisocoumarin
3-Acetamidocoumarin
6-FORMYLINDOLO [3,2-B] CARBAZOLE
7,7-DIMETHYLEICOSADIENOIC ACID
A-23187
Adiphenine hydrochloride
ADRENIC ACID (22:4, n-6)
AG-879
AGC
AGGC
Ajmaline
Albendazole
Alfadolone acetate
alpha-Santonin
Althiazide
AM-251
AM-580
Ambroxol hydrochloride
AMI-193
Amidopyrine
Amiloride hydrochloride dihydrate
Amoxicillin
ANANDAMIDE (20:3,n-6)
ANANDAMIDE (20:4, n-6)
ANANDAMIDE (22:4,n-6)
anisomycin
Antazoline hydrochloride
Antipyrine
Antipyrine, 4-hydroxy
ARACHIDONAMIDE
ARACHIDONIC ACID (20:4, n-6)
Arecoline hydrobromide
Articaine hydrochloride
Arvanil
Asiaticoside
Azaperone
Bambuterol hydrochloride
Benfluorex hydrochloride
Benperidol
Benzbromarone
Benzylpenicillin sodium
Betamethasone
Betaxolol hydrochloride
Biotin
Biperiden hydrochloride
Bisacodyl
BML-190
brefeldin A
Brinzolamide
BROMOCRIPTINE
Brompheniramine maleate
Buflomedil hydrochloride
Buspirone hydrochloride
Butoconazole nitrate
C2 CERAMIDE
capsazepine
Captopril
CARBACYCLIN
Carbetapentane citrate
Carbimazole
Carteolol hydrochloride
Cefixime
Ceforanide
Cefotiam hydrochloride
Cephaeline dihydrochloride heptahydrate
Cephalexin monohydrate
Cetirizine dihydrochloride
CGP 54626
CGP 55845
Chlorambucil
Chloramphenicol
Chlorogenic acid
Chlorprothixene hydrochloride
Chlorthalidone
CIGLITAZONE
CIMATEROL
Cimetidine
(-)-Cinchonidine
Ciprofibrate
CIRAZOLINE
Cisapride
Clebopride maleate
Clemizole hydrochloride
Clenbuterol hydrochloride
Clocortolone pivalate
Clopamide
Clorgyline hydrochloride
CLOTHIAPINE
Colchicine
Condelphine
Coralyne chloride hydrate
Corticosterone
Cyclacillin
Cyclobenzaprine hydrochloride
Cyclopenthiazide
Cyclopentolate hydrochloride
Cyclosporin A
Cyproheptadine hydrochloride
Cyproterone acetate
Dapsone
Daunorubicin hydrochloride
Dextromethorphan hydrobromide monohydrate
Dibucaine
DICHLOROBENZAMIL
Dicloxacillin sodium salt
Diflunisal
Digitoxigenin
Digoxin
DILTIAZEM
Diltiazem hydrochloride
Diprophylline
Dobutamine hydrochloride
DOMPERIDONE
Dosulepin hydrochloride
Doxylamine succinate
Droperidol
Dubinidine
Enilconazole
Enoxacin
Epirizole
ERGONOVINE
Ethamivan
Ethionamide
Etilefrine hydrochloride
Etoposide
Felodipine
FIPRONIL
Flucloxacillin sodium
FLUPHENAZINE
Fluticasone propionate
Fluvoxamine maleate
FPL-64176
Furosemide
Fursultiamine Hydrochloride
Fusaric acid
Fusidic acid sodium salt
Gabazine
GBR 12909 dihydrochloride
GBR 13069
Gibberellic acid
GINGEROL
Gliquidone
Glutethimide, para-amino
GR 103691
Griseofulvin
Guanfacine hydrochloride
Harmaline hydrochloride dihydrate
Harmol hydrochloride monohydrate
Harpagoside
Hydrochlorothiazide
Hydrocortisone base
Hydroflumethiazide
Hydroxytacrine maleate (R,S)
IB-MECA
Iobenguane sulfate
Iopanoic acid
Isoxicam
Isradipine
Kaempferol
Ketanserin tartrate hydrate
Ketotifen fumarate
L-741,626
Labetalol hydrochloride
Lansoprazole
L-cis-DILTIAZEM
LE 300
Letrozole
Leucomisine
Levamisole hydrochloride
Levocabastine hydrochloride
Lidoflazine
Lumicolchicine gamma
Luteolin
Maprotiline hydrochloride
MDL-28170
Meclozine dihydrochloride
Melatonin
Mephenytoin
Meprylcaine hydrochloride
Meptazinol hydrochloride
MESULERGINE
Methacycline hydrochloride
Methapyrilene hydrochloride
Methiazole
Methiothepin maleate
Methoxy-6-harmalan
Methoxy-8-psoralen
METHOXY VERAPAMIL
Meticrane
Metixene hydrochloride
METOCLOPRAMIDE
Miconazole
Minoxidil
MINOXIDIL
Mitoxantrone dihydrochloride
Molindone hydrochloride
Moricizine hydrochloride
Moxisylyte hydrochoride
Moxonidine
Myricetin
Nafronyl oxalate
Naftifine hydrochloride
Naftopidil dihydrochloride
Naphazoline hydrochloride
Naproxen
Naringenine
Naringin hydrate
Neostigmine bromide
Nicardipine hydrochloride
Nicergoline
Niclosamide
Nifurtimox
NIGULDIPINE
Niridazole
NITRENDIPINE
Nitrocaramiphen hydrochloride
Nitrofural
NO-711
Nomegestrol acetate
Norethynodrel
Omeprazole
Ondansetron Hydrochloride
Oxaprozin
Oxprenolol hydrochloride
Oxyphenbutazone
Ozagrel hydrochloride
Parbendazole
PCO-400
(+)-PD 128907
PHENAMIL
Phenazopyridine hydrochloride
Phensuximide
PHENTOLAMINE
Pilocarpine nitrate
Pindolol
Piperidolate hydrochloride
Piperlongumine
Piretanide
Piribedil hydrochloride
Pirlindole mesylate
Pizotifen malate
Practolol
Prednicarbate
PROCAINAMIDE
Procainamide hydrochloride
Procaine hydrochloride
Progesterone
Proguanil hydrochloride
Pronethalol hydrochloride
Propafenone hydrochloride
Propoxycaine hydrochloride
Propylthiouracil
Proscillaridin A
Puromycin dihydrochloride
Pyrimethamine
Quinethazone
QUINIDINE
Quinidine hydrochloride monohydrate
QUININE
(-)-Quinpirole hydrochloride
Ramipril
Remoxipride Hydrochloride
Repaglinide
Reserpinic acid hydrochloride
Rifabutin
Rimexolone
Ritodrine hydrochloride
(R)-Propranolol hydrochloride
Saquinavir mesylate
SCH 23390
Scopoletin
Sertaconazole nitrate
S(-)Eticlopride hydrochloride
SKF 38393
(+ -)-SKF-82958
S(-)-LISURIDE
Sotalol hydrochloride
Spiperone
SR-95639A
S(-)-RACLOPRIDE
S(+)-TERGURIDE
Strophantine octahydrate
Sulfadiazine
Sulfadoxine
Sulfaphenazole
Sulfathiazole
Sulfisoxazole
Suloctidil
Tacrine hydrochloride hydrate
Terconazole
Testosterone propionate
TETRANDRINE
THAPSIGARGIN
Thiamphenicol
Thyroxine (L)
Tiapride hydrochloride
Tiaprofenic acid
Tocainide hydrochloride
Torsemide
Tosyl-Phe-CMK (TPCK)
Tracazolate hydrochloride
Trapidil
Trazodone hydrochloride
Triamterene
Trifluridine
TRIM
Tropisetron HCl
zardaverine
Zidovudine, AZT
Zoxazolamine
Z-prolyl-prolinal