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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:45355*  Cite 

Upon treatment with HGF/SF, cells close to the wound edge begin to accelerate and stretch, deforming to elongated morphology, followed by enhanced directional migration. Green represent acceleration and stretching, purple represents increased directional migration, and light blue the overlapping regions of acceleration, stretching and directional migration.

Technical Details

DA3 cells expressing the fluorescent protein mCherry were grown to 90% confluence in wells of 2 cm diameter in DMEM supplemented with 10% heat-inactivated FCS (Gibco-BRL). A scratch was generated using a 200 µl tip, and the cells were incubated with 80 ng ml-1 Met-Hepatocyte Growth Factor/Scatter Factor (HGF/SF) and subjected to time lapse confocal laser scanning microscopy (CLSM-510, Zeiss, Germany) for approximately 26 hrs, with images taken at 14.5 min intervals. The acquired differential interference contrast (DIC) channel of the time-lapse sequence (shown here) was used for the multi-cellular analysis; the red fluorescence channel was not used here, and is not shown. The analysis used local motion-estimation information to extract spatiotemporal measures for cell acceleration, stretching (strain rate), and directional migration. The coloured strips in the video were extracted from this analysis. These results are under review and a reference to full details will be given upon publication.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
mammary adenocarcinoma
Cell Line
Biological Context
Biological Process
collective cell migration
wound healing, spreading of cells
Assaf Zaritsky
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
time lapse microscopy
Image Mode
differential interference contrast microscopy
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 1024px 1.24µm
Y 1024px 1.24µm