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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:44701*  Cite 

Immunofluorescence image of human IMR90 lung fibroblasts stained for vinculin (green) and filamentous actin (magenta). Nuclei are stained blue. This image of untreated fibroblasts comes from a study of the changes in adhesion that accompany treatment to induce stem cells and can be used in hiPSC purification. See also Singh et al. 2013 Nat Meth 10:438-444. The image appeared in the May 2013 issue of the NIGMS Biomedical Beat which features noteworthy NIGMS-supported research.

Technical Details

IMR90 cells in culture were treated with a cytoskeleton stabilizing buffer, extracted briefly with 0.5% Triton X-100, fixed with 4% paraformaldehyde, and immunostained stained for vinculin using a secondary antibody conjugated with Alexa 488 (green). The actin cytoskeleton was stained with phalloidin conjugated with Alexa 555 (magenta). Nuclei were counterstained with DAPI (blue). Preparations were observed using a Nikon E400 fluorescence microscope with 20x PLANAPO objective lens, and recorded with a SPOT CCD camera. For further details, see A Singh et al. 2013 Adhesion strength-based, label-free isolation of human pluripotent stem cells. Nat Meth 10:438-444 and associated Supplementary Material.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
fibroblast of lung
Cell Line
Cellular Component
Biological Context
Biological Process
cell adhesion
Andres J Garcia
Ankur Singh
NIGMS Biomedical Beat
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
charge coupled device (CCD)
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
differences in adsorption or binding of stain
distribution of epitope
Visualization Methods
Alexa Fluor 555
Alexa Fluor 488
4',6-diamidino-2-phenylindole (DAPI)
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 600px ——
Y 600px ——