Fluorescence microscope image of a cryostat section of mouse tongue epithelium immuno-stained for all taste bud cells (red) type II taste bud cells (green)and counter-stained for DNA (blue). The image was selected for inclusion in the April 18th 2013 issue of the NIGMS Biomedical Beat that features noteworthy research results.
Anesthetized mice were perfused with 4% paraformaldehyde, tongue epithelium dissected out, frozen, and 8 micrometer cryostat sections cut. Sections were immuno-stained for all taste buds (red, anti-KCNQ1, Alexa 586) and type II taste buds (green, anti-TRPM5, Alexa 488), counter-stained with DAPI (blue), and recorded with a Zeiss LSM 710 confocal microscope equipped with 40x NA 1.1 water immersion objective lens. The image shows a single confocal plane with the red, green, and blue channels merged. See also: Akiyuki T et al. 2013. CALHM1 ion channel mediates purinergic neurotransmission of sweet, bitter and umami tastes. Nature 495:223-226.
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