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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:44152*  Cite 
Description

The picture represents a 3D-reconstruction (using Imaris software) of confocal/multiphoton images collected through the thickness (100 micrometers) of the live skin from a bone-marrow transplanted mouse. Cells marked by five fluorescent proteins: Cerulean (cyan), EGFP (green), Venus (yellow), tdTomato (purple), mCherry (red) appear interspersed along reticulin and collagen fibers (second harmonic generation-SHG, white) at 4 months post-transplant. See also Malide et al. (2012) Dynamic clonal analysis of murine hematopoietic stem and progenitor cells marked by 5 fluorescent proteins using confocal and multiphoton microscopy. Blood. Dec 20;120(26):e105-16. This image is grouped with two others obtained in a similar fashion.

Technical Details

Imaging was performed using a Leica TCS SP5-AOBS 5-channel confocal/two-photon system equipped with multiline Argon diode 561 nm, HeNe 594 nm and HeNe 633 nm as well as TiSaph two-photon laser. Images were recorded with a 25x 0.95 NA water dipping objective lens, and 5 micrometer z-spacing and processed using Imaris software (Bitplane). See also: Malide D, Metais J-Y, and Dunbar CE. (2012). Dynamic clonal analysis of murine hematopoietic stem and progenitor cells marked by 5 fluorescent proteins using confocal and multiphoton microscopy. Blood. Dec 20;120(26):e105-16.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
epidermal cell
endodermal cell
hair follicle
muscle cell
Cellular Component
cytoplasm
collagen fiber
elastin fiber
reticulin fiber
Biological Context
Biological Process
clonal expansion of progenitor cells
Attribution
Names
Daniela Malide
Jean-Yves Metais
Cynthia Dunbar
OTHER
National Institutes of Health, Bethesda MD
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL44152
Archival Resource Key (ARK)
ark:/b7295/w9cil44152
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
multi-photon microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
compartmentalization of stain or label
Visualization Methods
CeruleanFP
EGFP
VenusFP
tdTomatoFP
mCherryFP
second harmonic generation - SHG
Data Qualifiers
processed data
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 939px ——
Y 952px ——