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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:41729*  Cite 
Description

High Voltage EM image of CHO metaphase chromosomes from a 'thick' section of metaphase-arrested cells treated with D20 to stabilize microtubules. This image was recorded with a specimen tilt of 55 degrees. The accompanying grouped image had a tilt of 45 degrees to create a stereo pair providing an oblique 3D view of the chromosomes.

Technical Details

CHO cells at metaphase were treated with 50% D2O, fixed with glutaraldehyde, embedded in epoxy resin and 0.5 micrometer 'thick' sections stained with uranyl acetate and lead citrate for HVEM observation at 1 MeV. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Biological Sources
NCBI Organism Classification
Cricetulus griseus
Cell Type
ovary
Cell Line
CHO
Cellular Component
nuclear chromosome
Biological Context
Biological Process
mitosis
mitotic metaphase
chromosome organization
Attribution
Names
Hans Ris
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL41729
Archival Resource Key (ARK)
ark:/b7295/w9cil41729
Grouping This image is part of a group.
Imaging
Image Type
film
Image Mode
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
chemically fixed tissue
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 2824px 1nm
Y 4220px 1nm