The Cell: An Image Library - Image CIL:41711

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CIL:41711^*

Description

High Voltage (1MeV) EM image of a metaphase chromosome isolated from CHO cells using a non-aqueous technique, squashed in 1:1 acetic acid:ethanol and transferred to an EM grid. A chromatid pair occupies the center of the image. This micrograph was captured at a specimen tilt of 55 degrees. The accompanying image has a tilt of 45 degrees, creating a stereo pair that provides an oblique stereo view of the chromatids.

Technical Details

Cells were metaphase arrested with colcemid, then exposed to a low ionic strength medium (0.07M phosphate buffer)containing 50% D20 as a microtubule stabilizer. Preparations were squashed in 3:1 acetic acid:ethanol,stained, and transferred to an EM grid. See also: H. Ris 1981 Stereoscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Biological Sources

NCBI Organism Classification: Cricetulus griseus
Cell Type: ovary
Cell Line: CHO
Cellular Component: nuclear chromosome

Biological Context

Biological Process: mitosis; mitotic metaphase; chromosome organization

Attribution

Name: Hans Ris
Date: 07/24/1977

Grouping

This image is part of a group.

Imaging

Image Type: film
Imaging Mode: FBbi:00000622
Parameters Imaged: electron density
Source of Contrast: differences in adsorption or binding of stain
Visualization Methods: uranyl salt
Processing History: unprocessed raw data

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: chemically fixed tissue
Relation To Intact Cell: squash

Dimensions

Spatial Axis	Image Size	Pixel Size
X	4322px	1.0nm
Y	6628px	1.0nm

*CIL – Cell Image Library accession number. Please use this to reference an image.