Licensing
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Comments:
Description
Confocal micrograph of NIH-3T3 cells co-transduced with 5 fluorescent proteins. The cells were marked by co-transduction with Lentiviral Gene Ontology (LeGO) vectors expressing Cerulean (cyan), EGFP (green), Venus (yellow), tdTomato (magenta) or mCherry (red) fluorescent proteins to provide combinatorial colors for cell lineage tracing. These are cytosolic-untargeted FPs (just to mark the cells). The image was collected on live cells with a Leica SP5 confocal system. Honorable Mention, 2011 Olympus BioScapes Digital Imaging Competition®.
Technical Details
Biological Sources
- NCBI Organism Classification
- Mus musculus
- (house mouse)
- Cell Type
- fibroblast
- Cell Line
- NIH/3T3
- Cellular Component
- cytoplasm
Attribution
- Names
- Daniela Malide
- 2011 Olympus BioScapes Digital Imaging Competition®
- Link
- 2011 Olympus BioScapes Digital Imaging...
- Other
- National Institutes of Health, Bethesda, MD, USA
Imaging
- Image Type
- recorded image
- Imaging Mode
- confocal microscopy
- Parameters Imaged
- fluorescence emission
- Source of Contrast
- compartmentalization of stain or label
- Visualization Methods
- CeruleanFP
- EGFP
- VenusFP
- tdTomatoFP
- mCherryFP
Sample Preparation
- Methods
- living tissue
- Relation To Intact Cell
- dispersed cells in vitro
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 1976px | —— |
| Y | 1966px | —— |
*CIL – Cell Image Library accession number. Please use this to reference an image.