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CIL:41403*  Cite 

Video of an array tomogram of a mouse somatosensory cortex. The volume is a "fly-through" of a sagitally oriented rectangular slab of transgenic mouse somatosensory cortex. The overlay thumbnail at upper left renders a projection through the entire slab and the moving magenta rectangle shows the variably smaller area rendered full screen. The rendered volume was acquired by array tomography and stitched together in three dimensions from >10,000 image tiles. Green in this movie renders native FFP fluorescence, red renders anti-synapsin 1 immunofluorescence, and blue renders anti-tubulin immunofluorescence. Dr. Smith wishes to acknowledge the work of Kristina Micheva, Brad Busse, Nicholas Weiler, Nancy O'Rourke and Gordon Wang. This video does not have audio on the Olympus BioScapes website, but a score by Catherine Rose Smith has been composed and performed to accompany the video. More information is provided in two articles: Micheva, K.D., Busse, B.L., Weiler, N.C., O'Rourke, N., Smith, S.J (2010), Neuron 68:639-653; and Micheva, K.D., and Smith, S.J (2007), Neuron 55:25-36. Judges' Special Award for Technical Merit, 2011 Olympus BioScapes Digital Imaging Competition®.

Technical Details

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
Cellular Component
microtubule cytoskeleton
synapse part
Biological Context
Biological Process
somatosensory cortex morphogenesis
Mouse Gross Anatomy
somatosensory cortex
Stephen Smith
2011 Olympus BioScapes Digital Imaging Competition®
2011 Olympus BioScapes Digital Imaging Compet...
Stanford University, Stanford, CA USA
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
array tomography
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
distribution of a specific protein
Visualization Methods
primary antibody plus labeled secondary antibody
Processing History
see details listed below
Sample Preparation
formaldehyde fixed tissue
microwave fixation
ethanol dehydration
LRWhite resin
Relation To Intact Cell
microtome-sectioned tissue
Spatial Axis Image Size Pixel Size
X 960px ——
Y 540px ——