Total internal reflection (TIRF) micrograph of MDCK cells stably transfected with the apical membrane protein P75-GFP (green) and stained for acetylated tubulin of the primary cilium (red) by immunofluorescence.
Cells were grown for 5 days prior to imaging with apical TIRF microscopy. Images were taken with a Leica AM TIRF MC. Penetration depth: 90 nmImage collected using a True MultiColor Laser TIRF Leica AM TIRF MC. See http://www.leica-microsystems.com/science-lab/protein-transport-processes-at-the-apical-membrane-of-polarized-epithelial-cells-applications-for-tirf-microscopy/ for additional details.
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