Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:41313^* Cite

Description

Total internal reflection (TIRF) micrograph of MDCK cells stably transfected with the apical membrane protein P75-GFP (green) and stained for acetylated tubulin of the primary cilium (red) by immunofluorescence.

Technical Details

Cells were grown for 5 days prior to imaging with apical TIRF microscopy. Images were taken with a Leica AM TIRF MC. Penetration depth: 90 nmImage collected using a True MultiColor Laser TIRF Leica AM TIRF MC. See http://www.leica-microsystems.com/science-lab/protein-transport-processes-at-the-apical-membrane-of-polarized-epithelial-cells-applications-for-tirf-microscopy/ for additional details.

Biological Sources

NCBI Organism Classification: Canis lupus familiaris
Cell Type: epithelial cell
Cell Line: MDCK-II
Cellular Component: primary cilium; acetylated tubulin; P-75

Attribution

Names: Christoph Greb; Ralf Jacob; Anja Schu

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL41313
Archival Resource Key (ARK): ark:/b7295/w9cil41313

Imaging

Image Type: recorded image
Image Mode: total internal reflection microscopy (TIRF)
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of epitope; distribution of a specific protein
Visualization Methods: EGFP; primary antibody plus labeled secondary antibody

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	709px	——
Y	708px	——