Licensing

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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40984^* Cite

Description

Montage image of a brain stem from a Brainbow transgenic mouse. In Brainbow mice, neurons randomly choose combinations of red, yellow and cyan fluorescent proteins, so that they each glow a particular color. This provides a way to distinguish neighboring neurons and visualize brain circuits. These are large caliber axons of the auditory pathway. First Prize, 2007 Olympus BioScapes Digital Imaging Competition. For additional details see: Livet J, Weissman TA, Kang H, Draft RW, Lu J, Bennis RA, Sanes JR, Lichtman JW. Nature. 2007 Nov 1;450(7166):56-62.

Technical Details

Biological Sources

NCBI Organism Classification: Mus musculus
Cell Type: neuron
Cellular Component: neuron projection

Biological Context

Biological Process: neuron projection morphogenesis
Mouse Gross Anatomy: brain stem

Attribution

Names: Jean Livet; 2007 Olympus BioScapes Digital Imaging Competition
Link: 2007 Olympus BioScapes Digital Imaging Compet...
OTHER: Jeff Lichtman Laboratory, Harvard University Cambridge, MA, USA

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL40984
Archival Resource Key (ARK): ark:/b7295/w9cil40984

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: confocal microscopy
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: Yellow fluorescent proteins from Aequorea; Cyan fluorescent proteins from Aequorea; Red fluorescent proteins from Anthozoa

Sample Preparation

Methods: formaldehyde fixed tissue
Relation To Intact Cell: sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1737px	——
Y	1258px	——