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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40952*  Cite 
Description

Colorized transmission electron micrograph of a platinum replica showing the cytoskeletal organization of stubby dendritic spines in extracted hippocampal neurons after 14 DIV. Unlabeled inset shows the smaller version of the corresponding main panel with axons color-coded in purple, dendrites in yellow, and spines in cyan. Yellow box indicates the enlarged panel shown on the bottom of the image. This box shows branched actin filaments (cyan) in the spine bases associate with dendritic microtubules (red). Bar, 0.2 μm. Image corresponds to Figure 3b in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel (this image) is available as CIL 40952. The uncolorized image is available as CIL 40804, and the insert is available as CIL 40810.

Technical Details

Please refer to referenced article for details on cell extraction and processing for electron microscopy.

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
hippocampal neuron
Cellular Component
axon
dendrite
dendritic spine
actin cytoskeleton
microtubule cytoskeleton
Biological Context
Biological Process
dendrite morphogenesis
Attribution
Names
Farida Korobova
Tatyana Svitkina
Published
Mol Biol Cell. 2010 Jan 1;21(1):165-76. Epub 2009 Nov 4.
Pubmed
19889835
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40952
Archival Resource Key (ARK)
ark:/b7295/w9cil40952
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
Processing History
colorized
Dimensions
Spatial Axis Image Size Pixel Size
X 572px ——
Y 1236px ——