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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40701*  Cite 

High voltage (1MeV) transmission electron microscopy image of an isolated metaphase chromatid pair from a Chinese hamster ovary cell, showing the fiber-like structure and two large pieces of attached membrane. The image was taken with a specimen tilt of 55 degrees. Grouped with it is an image with a tilt of 48 degrees, providing a pair that affords an oblique stereo view of the chromosome.

Technical Details

Cells were treated with colchicine for 24 hr, and metaphase chromosomes isolated using the non-aqueous hexylene glycol protocol developed by Stubblefield and Wray, placed on a grid, critical point dried, and stained with uranyl acetate. See also: H. Ris 1981 Steroscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Biological Sources
NCBI Organism Classification
Cricetulus griseus
Cell Type
epithelial cell
Cell Line
Cellular Component
nuclear chromosome
Biological Context
Biological Process
chromosome organization
mitotic metaphase
Hans Ris
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
Image Mode
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Spatial Axis Image Size Pixel Size
X 4700px 1nm
Y 6950px 1nm