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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40684*  Cite 

High voltage (1MeV) transmission electron microscopy image of an isolated metaphase chromatid pair from a mouse A9 cultured cell, showing the fiber-like structure and several pieces of attached membrane. The image was taken with a specimen tilt of 46 degrees. Grouped with it is an image with a tilt of 53 degrees, providing a pair that affords an oblique stereo view of the chromosome.

Technical Details

Chromosomes were isolated from mouse A9 cells arrested in metaphase using a non-aqueous protocol, placed on a grid, and stained with uranyl acetate. See also: H. Ris 1981 Steroscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
Cell Line
Cellular Component
nuclear chromosome
Biological Context
Biological Process
chromosome organization
mitotic metaphase
Hans Ris
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
Image Mode
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Spatial Axis Image Size Pixel Size
X 3597px 1.27nm
Y 5520px 1.27nm