High voltage (1MeV) transmission electron microscopy image of an isolated metaphase chromatid pair from a mouse A9 cultured cell, showing the fiber-like structure and several pieces of attached membrane. The image was taken with a specimen tilt of 53 degrees. Grouped with it is an image with a tilt of 46 degrees, providing a pair that affords an oblique stereo view of the chromosome.
Chromosomes were isolated from mouse A9 cells arrested in metaphase using a non-aqueous protocol, placed on a grid, and stained with uranyl acetate. See also: H. Ris 1981 Steroscopic electron microscopy of chromosomes. Meth Cell Biol 22:77-96 H. Ris 1978 Preparation of chromatin and chromosomes for electron microscopy. Meth Cell Biol 18:220-246.
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