Platinum replica illustrating the cytoskeletal organization of dendritic spines from extracted 14 DIV neurons. Thin spines associate with dendrites at the base (bottom) and with axons by the head (top). Unlabeled insets show the smaller versions of the corresponding main panels with axons color-coded in purple, dendrites in yellow, and spines in cyan. Thick fibers in neurites represent microtubules. Yellow boxes are enlarged in panels with corresponding numbers. Box 4, interaction of actin filaments (cyan) with an axonal microtubule (red). Box 5, branched actin filaments (cyan) in the neck of respective spines. This image is part of a group of images representing Figure 2B and its components from Mol Biol Cell. 2010 Jan 1;21(1):165-76. Figure 2B (this image) appears as CIL 40664. The uncolorized overview panel is CIL 40667. Box 4 appears as CIL 40665, and Box 5 appears as CIL 40666.
Please refer to referenced article for details on cell extraction and processing for electron microscopy.
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