Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. This image shows branched actin filaments (cyan) at the neckâ€“head junction. Dashed arrows indicate potential filament breakage. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel is available as CIL 40660. The uncolorized image is available as CIL 40659. Box 1, 2, and 3 (this image) are CIL 40661, 40662, and 40663, respectively.
Please refer to referenced article for details on cell extraction and processing for electron microscopy.