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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40660*  Cite 
Description

Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. Mushroom spines associate with dendrites at the base (bottom) and with axons by the head (top). Unlabeled insets show the smaller versions of the corresponding main panels with axons color-coded in purple, dendrites in yellow, and spines in cyan. Thick fibers in neurites represent microtubules. Red asterisks indicate putative PSD fragments. Yellow boxes are enlarged in panels with corresponding numbers. Box 1, interaction of putative PSD (arrow) from the spine head with axonal intermediate filaments (green); actin filaments are shown in cyan and a microtubule in red. Boxes 2 and 3, branched actin filaments (cyan) in the head (2) and at the neck–head junction (3). Insets in panels 2 and 3 show nonpseudocolored regions outlined by yellow boxes. Dashed arrows in panel 3 indicate potential filament breakage. Bars, 0.2 μm. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel (this image) is available as CIL 40660. The uncolorized image is available as CIL 40659. Box 1, 2, and 3 are CIL 40661, 40662, and 40663, respectively.

Technical Details

Please refer to referenced article for details on cell extraction and processing for electron microscopy.

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
hippocampal neuron
Cellular Component
dendritic spine
dendritic shaft
post synaptic density
actin cytoskeleton
microtubule cytoskeleton
intermediate filament cytoskeleton
Biological Context
Biological Process
dendritic spine organization
dendrite morphogenesis
Attribution
Names
Farida Korobova
Tatyana Svitkina
Published
Mol Biol Cell. 2010 Jan 1;21(1):165-76. Epub 2009 Nov 4.
Pubmed
19889835
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40660
Archival Resource Key (ARK)
ark:/b7295/w9cil40660
Grouping This image is part of a group.
Imaging
Image Type
grey scale graphic
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
shadowing and plating
Processing History
colorized
Dimensions
Spatial Axis Image Size Pixel Size
X 894px ——
Y 2587px ——