Platinum replica depicting the cytoskeletal organization of dendritic spines in extracted 14 DIV neurons. Mushroom spines associate with dendrites at the base (bottom) and with axons by the head (top). Thick fibers in neurites represent microtubules. Image corresponds to Figure 2a in Mol Biol Cell. 2010 Jan 1;21(1):165-76. The entire panel is available as CIL 40660. The uncolorized image (this image) is available as CIL 40659. Box 1, 2, and 3 are CIL 40661, 40662, and 40663, respectively.
Please refer to referenced article for details on cell extraction and processing for electron microscopy.