Licensing
Description
The 11 micrographs of the group are views of a serially-sectioned contracted Vorticella convallaria cell that show the main features of this cell. This figure shows 3 food vacuoles; 2 segments of the macronucleus; micronucleus; perioral cilia; tips of “ribbed wall” along adoral side of the peristome that contain the lamellae; paroral cilia; contractile vacuole; tube or chamber containing bacteria linking the contractile vacuole to the peristome; and myonemes linking the perioral zone to the cell's lateral pellicle. TEM taken on 4/1/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 2,150X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Technical Details
Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
Biological Sources
- NCBI Organism Classification
- Vorticella convallaria
- Cell Type
- cell by organism
- eukaryotic cell
- Eukaryotic Protist
- Ciliated Protist
- Cell Line
- Cambridge Culture Collection, Cambridge, England, # LB1690/1
- Cellular Component
- cell
- food vacuole
- macronucleus
- micronucleus
- oral apparatus
- contractile vacuole
Biological Context
Attribution
- Name
- Richard Allen (University of Hawaii)
- Link
- http://www5.pbrc.hawaii.edu/allen/
Grouping
This image is part of a group.Imaging
- Image Type
- transmission electron microscopy (TEM)
- illumination by electrons
- Imaging Mode
- detection of electrons
- film
- Parameters Imaged
- electron density
- Source of Contrast
- stain with broad specificity
- Visualization Methods
- stain with broad specificity
- osmium tetroxide
- uranyl salt
- lead salt
- Processing History
- recorded image
- film
- Print from negative scanned for Photoshop.
- Data Qualifiers
- processed data
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- osmium tetroxide fixed tissue
- tissue in epoxy resin embedment
- microtome-sectioned tissue
- Relation To Intact Cell
- microtome-sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 3624px | 3.7nm |
| Y | 4000px | 3.7nm |