The Cell: An Image Library - Image CIL:40570

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CIL:40570^*

Description

The 11 micrographs of the group are views of a serially-sectioned contracted Vorticella convallaria cell that show the main features of this cell. This figure shows 1 food vacuole; micronucleus; contractile vacuole connected by 2 CV pores to the bacteria-filled chamber; peristome; perikinetids of the perioral cilia. TEM taken on 4/1/71 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 3,400X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Technical Details

Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources

NCBI Organism Classification: Vorticella convallaria
Cell Type: cell by organism; eukaryotic cell; Eukaryotic Protist; Ciliated Protist
Cell Line: Cambridge Culture Collection, Cambridge, England, # LB1690/1
Cellular Component: cell; food vacuole; micronucleus; contractile vacuole; contractile vacuole pore; oral apparatus; food vacuole

Biological Context

Biological Process: digestive system process; cytoplasm organization; cortical cytoskeleton organization; micronucleus organization; contractile vacuole organization; oral apparatus organization; digestive system process

Attribution

Name: Richard Allen (University of Hawaii)
Link: http://www5.pbrc.hawaii.edu/allen/

Grouping

This image is part of a group.

Imaging

Image Type: transmission electron microscopy (TEM); illumination by electrons
Imaging Mode: detection of electrons; film
Parameters Imaged: electron density
Source of Contrast: stain with broad specificity
Visualization Methods: stain with broad specificity; osmium tetroxide; uranyl salt; lead salt
Processing History: recorded image; film

Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: glutaraldehyde fixed tissue; osmium tetroxide fixed tissue; tissue in epoxy resin embedment; microtome-sectioned tissue
Relation To Intact Cell: microtome-sectioned tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	3710px	2.4nm
Y	4000px	2.4nm

*CIL – Cell Image Library accession number. Please use this to reference an image.