Detail of acidosome docking sites at DV-IA planar view through the site where acidosomes are docked and/or fusing with a DV-I. These sites are labeled for the mAb specific for antigen L1. TEM taken on 9/27/93 by R. Allen with Zeiss 10A operating at 80kV. Neg. 15,900X. Published in J. Cell Sci. 108:1263-1274, 1995, and adapted with permission.
To label membranes inside the cell we used very lightly fixed cells (0.25% glutaraldehyde) that were then rapidly frozen in liquid nitrogen and sectioned later at -100oC. These frozen sections were picked up on drops of methylcellulose and transferred to a Formvar-supported grid. The sections were immunogold labeled (15nm gold) to show the location of the specific antigen inside the cell as well as on the cell surface. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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