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CIL:40556*  Cite 

Monoclonal antibody, E30-2-1, was raised to antigen D6 that specifically labels acidosomes and DV-II. There was no label on the nascent vacuole membrane or ciliary membranes. This cell had been incubated in 1.1µm latex beads for 3 minutes and fixed without a chase. There is some gold over the cytosol but the nascent vacuole lumen is essentially free of label. TEM taken on 7/12/90 by R. Allen with Zeiss 10A operating at 80kV. Neg. 9,780X. Adapted with permission.

Technical Details

To label membranes inside the cell we used very lightly fixed cells (0.25% glutaraldehyde) that were then rapidly frozen in liquid nitrogen and sectioned later at -100oC. These frozen sections were picked up on drops of methylcellulose and transferred to a Formvar-supported grid. The sections were immunogold labeled (15nm gold) to show the location of the specific antigen inside the cell as well as on the cell surface. Microtome sections prepared at approximately 75nm thickness. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
food vacuole
early phagosome
Biological Context
Biological Process
digestive system process
Richard Allen (University of Hawaii)
J Cell Sci. 1995 Mar;108 ( Pt 3):1263-74.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
intrinsic mass distribution
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
uranyl salt
Processing History
recorded image
Print from negative scanned for Photoshop.
Data Qualifiers
processed data
Sample Preparation
glutaraldehyde fixed tissue
tissue in vitreous ice embedment
microtome-sectioned tissue
monoclonal antibody
Relation To Intact Cell
microtome-sectioned tissue
Spatial Axis Image Size Pixel Size
X 4985px 1.5nm
Y 5712px 1.5nm