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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40505*  Cite 
Description

High magnification view of a crushed mitochondrion showing the zipper-like row of 9nm diameter particles on the outer curve of the cristae and the single row of larger particles on the inner curve. There is an average of 1500 particles per square micrometer of 50-nm tubular cristae surface on the outer curve. These particles are thought to be the F1 units of the F1F0 ATP synthases of the mitochondria. The larger particles on the inner curve resemble complex I of the respiratory electron transport chain in size and relative abundance when compared to their relative quantities estimated from biochemical studies in other cells such as beef heart. This shows the highly ordered arrangement of some of the molecules of the oxidative phosphorylation and electron transport systems in Paramecium. TEM taken on 5/6//88 by C. Schroeder with Zeiss 10A operating at 80kV. Neg. 63,000X. The raw negative was scanned with an Epson Perfection V750 Pro and this high resolution image is best used for quantitative analysis. Additional information available at (http://www5.pbrc.hawaii.edu/allen/). Published in J. Cell Biol. 108:2233-2240, 1989. Adapted with permission.

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
mitochondrial proton-transporting ATP synthase complex, catalytic core F(1)
mitochondrion
Biological Context
Biological Process
regulation of mitochondrion organization
Attribution
Names
Richard Allen (University of Hawaii)
C. Schroeder
Published
J Cell Biol. 1989 Jun;108(6):2233-40.
Pubmed
2525561
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40505
Archival Resource Key (ARK)
ark:/b7295/w9cil40505
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
electron density
Parameters Imaged
elastic scattering of electrons
electron density
elevation
Source of Contrast
differences in deposition of metal shadow
Visualization Methods
transmission electron microscopy (TEM)
Processing History
freeze_fracture/freeze_etch
shadowing and plating
recorded image
film
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 3726px 0.32nm
Y 4048px 0.32nm