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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40464*  Cite 
Description

Single slice confocal images representing one optical slice of a cell stained for the Human Protein Atlas (HPA) antibody HPA017340 (shown in green). HPA017340 is an anti-LEMD2 antibody that labels the nuclear membrane. The cells are also stained with reference markers in order to facilitate the annotation of the subcellular distribution of the protein targeted by the HPA antibody. The following probes/organelles are used as references; (i) DAPI for the nucleus (blue) and (ii) anti-tubulin antibody as internal control and marker of microtubules (red).

Technical Details

The use of data and images from this site in publications and presentations is permitted provided that the following conditions are met: The publication and/or presentation are solely for informational and non-commercial purposes. The source of the data and/or image is referred to this site (www.proteinatlas.org) and/or the above publication is cited.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
glioma
Cell Line
U-251
Cellular Component
nuclear membrane
nucleus
microtubule cytoskeleton
Biological Context
Biological Process
organelle organization
cytoskeleton organization
Attribution
Names
M Uhlen
P Oksvold
L Fagerberg
E Lundberg
K Jonasson
M Forsberg
M Zwahlen
C Kampf
K Wester
S Hober
H Wernerus
L Björling
F Ponten
Published
Nat Biotechnol. 2010 28(12):1248-50.
Pubmed
21139605
Link
Human Protein Atlas
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40464
Archival Resource Key (ARK)
ark:/b7295/w9cil40464
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
chemically fixed tissue
permeabilized tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1728px ——
Y 1728px ——