Transmission electron micrograph of a representative untreated swa2 mutant cell. SWA2 encodes the yeast homolog of auxilin, which is a co-chaperone that regulates clathrin assembly and disassembly. swa2 mutant cells exhibit a vacuolar fragmentation phenotype. (6,300X). An image of the same mutant following sertraline treatment is available as CIL 40435.
This group of images contains representative whole-cell images and high-magnification zooms of organelle structures of interest. All cells were grown in liquid rich media (YPD pH6.5) at 30C with aerated shaking, and cultures were sampled in mid-logarithmic phase. Treated cells were exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.
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