Fluorescence images showing the distribution of GFP-ALADIN, a WD-repeat protein that is mutated in the human triple A syndrome (green), and the nucleoporin Nup358(red)within the nuclear envelope of HeLa cells. The two proteins show a close overlap with Nup358 slightly more distal to the midplane of the nuclear pore complex. Other images in this group show the ultrastructural effects of mutant ALADIN.
HeLa cells expressing GFP-ALADIN were fixed with formaldehyde immunostained for Nup358 and observed with a Delta-vision deconvolution system. See: J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827
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