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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40312*  Cite 
Description

Fluorescence images showing the distribution of GFP-ALADIN, a WD-repeat protein that is mutated in the human triple A syndrome (green), and the nucleoporin Nup358(red)within the nuclear envelope of HeLa cells. The two proteins show a close overlap with Nup358 slightly more distal to the midplane of the nuclear pore complex. Other images in this group show the ultrastructural effects of mutant ALADIN.

Technical Details

HeLa cells expressing GFP-ALADIN were fixed with formaldehyde immunostained for Nup358 and observed with a Delta-vision deconvolution system. See: J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
cervical carcinoma
Cell Line
HeLa
Cellular Component
nucleus
nuclear envelope
nuclear pore
Attribution
Names
Janet Cronshaw
Michael Matunis
Published
J Cronshaw and M Matunis 2003 The nuclear pore complex ALADIN is mislocalized in triple A syndrome Proc Natl Acad Sci USA 100 5823-5827
Pubmed
12730363
Link
Proceedings National Academy of Sciences USA
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40312
Archival Resource Key (ARK)
ark:/b7295/w9cil40312
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
wide-field detection
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Alexa Fluor 488
Alexa Fluor 594
EGFP
Data Qualifiers
processed data
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 699px ——
Y 549px ——