Licensing
Description
Transmission electron micrograph of a yeast cell exposed to 60µM antidepressant sertraline. This high-magnification zoom of CIL 39708 shows a double-membrane autophagosome. Encapsulated inside the autophagosomes is a multilamellar membrane “whorl.”
Technical Details
BY4716 is an auxotrophic haploid laboratory strain of the budding yeast Saccharomyces cerevisiae. Its genotype is lys2Δ, mating type alpha and it is derived from S288c. Sections were embedded in LR white resin, mounted on carbon-coated nickel grids and imaged with LEO 912AB at 80kV and 6,300X. All cells in this batch were grown in liquid rich media (YPD pH6.5) at 30?C with aerated shaking, and cultures were sampled in mid-logarithmic phase. This image is part of a group of untreated cells and cells exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.
Biological Sources
- NCBI Organism Classification
- Saccharomyces cerevisiae
- (Baker's Yeast, Brewer's Yeast)
- Cell Type
- BY4716
- Cellular Component
- autophagic vacuole
- autophagic vacuole membrane
- autophagic vacuole lumen
Biological Context
- Biological Process
- organelle organization
- sertraline treatment
Attribution
- Names
- Ethan Perlstein
- Daniel Korostyshevsky
- Other
- Princeton EM Core Facility
Grouping
This image is part of a group.Imaging
- Image Type
- recorded image
- Imaging Mode
- transmission electron microscopy (TEM)
- Parameters Imaged
- electron density
- Source of Contrast
- differences in adsorption or binding of stain
- Visualization Methods
- osmium tetroxide
- Processing History
- unprocessed raw data
Sample Preparation
- Methods
- glutaraldehyde fixed tissue
- tissue in plastic embedment
- Relation To Intact Cell
- sectioned tissue
Dimensions
| Spatial Axis | Image Size | Pixel Size |
|---|---|---|
| X | 2115px | —— |
| Y | 2500px | —— |