Alternate header for print version


Licensing
Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40306*  Cite 
Description

Transmission electron micrograph of a yeast cell exposed to 60µM antidepressant sertraline. This high-magnification zoom of CIL 39708 shows a double-membrane autophagosome. Encapsulated inside the autophagosomes is a multilamellar membrane “whorl.”

Technical Details

BY4716 is an auxotrophic haploid laboratory strain of the budding yeast Saccharomyces cerevisiae. Its genotype is lys2Δ, mating type alpha and it is derived from S288c. Sections were embedded in LR white resin, mounted on carbon-coated nickel grids and imaged with LEO 912AB at 80kV and 6,300X. All cells in this batch were grown in liquid rich media (YPD pH6.5) at 30˚C with aerated shaking, and cultures were sampled in mid-logarithmic phase. This image is part of a group of untreated cells and cells exposed to 60µM antidepressant sertraline (Zoloft®) for 45 minutes.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae
Cell Type
BY4716
Cellular Component
autophagic vacuole
autophagic vacuole membrane
autophagic vacuole lumen
Biological Context
Biological Process
organelle organization
sertraline treatment
Attribution
Names
Ethan Perlstein
Daniel Korostyshevsky
OTHER
Princeton EM Core Facility
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40306
Archival Resource Key (ARK)
ark:/b7295/w9cil40306
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
Processing History
unprocessed raw data
Sample Preparation
Methods
glutaraldehyde fixed tissue
tissue in plastic embedment
Relation To Intact Cell
sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 2115px ——
Y 2500px ——