Time lapse movie showing the change in microtubule distribution in cultured porcine kidney epithelial cells (LLCPK1) stably expressing GFP-alpha tubulin during the progression from prophase to prometaphase. The cytoskeleton is reorganized with disassembly of individual microtubules and formation of microtubule bundles, causing an inward collapse of the microtubule array prior to the formation of the mitotic spindle.
Living LLCPK1 cells expressing GFP-alpha-tubulin were observed using a Nikon Eclipse TE 300 microscope equipped with a 100x phase NA 1.4 objective lens, and images captured at 2 sec intervals with a Perkin Elmer spinning disc confocal scan head and a cooled CCD camera See also supplemental video 1 in: NM Rusan et al. 2002 Reorganization of the microtubule array in prophase/prometaphase requires cytoplasmic dynein-dependent microtubule transport. J Cell Biol 158:997-1003.
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