Maximum intensity projection of an optical section series of a protoplasmic astrocyte injected with Lucifer Yellow from the hippocampal area CA1 in a 1 week old male rat. For more information, see: Bushong et al. Maturation of astrocyte morphology and the establishment of astrocyte domains during postnatal hippocampal development. Int J Dev Neurosci. 2004 Apr;22(2):73-86. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.
Tissue was sectioned at a thickness of 100 µm with vibratome. Optical sections (at intervals of 0.2 µm) were gathered using a Biorad Radiance 2000 Confocal, 60X N.A. 1.4 oil-immersion objective.