Alternate header for print version


Licensing
Attribution Only: This image is licensed under a Creative Commons Attribution License. View License Deed | View Legal Code
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40257*  Cite 
Description

A single image from animation of the computed slices through a tomographic volume of mitochondria at the Node of Ranvier in rat peripheral nerve root. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information, see: Perkins et al. Mitochondrial configurations in peripheral nerve suggest differential ATP production. J. Struct. Biol. (2010), doi:10.1016/j.jsb.2010.06.017 http://www.ncbi.nlm.nih.gov/pubmed/20600951

Technical Details

Chemical fixation with 2% paraformaldehyde and 2.5% glutaraldehyde via vascular perfusion in 0.15M cacodylate buffer (pH 7.4) at 37 degrees C Neuronal sections cut to a thickness of 300-500 nm were taken on 100 mesh copper clamshell grids. The sections on the grid were stained for 30 minutes to 60 minutes in 2% aqueous uranyl acetate then approximately 15 min in lead salts. 20nm fiducial markers were placed on the specimen.

Biological Sources
NCBI Organism Classification
Rattus norvegicus
Cell Type
peripheral neuron
Cellular Component
mitochondrion
node of Ranvier
Attribution
Names
Mark H. Ellisman
Guy A. Perkins
Link
CCDB 8141
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40257
Archival Resource Key (ARK)
ark:/b7295/w9cil40257
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
Parameters Imaged
electron density
Data Qualifiers
processed data
Dimensions