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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40249*  Cite 

A single frame from a time-lapse animation of FlAsH-labeled MDCK cells expressing Cx43-4C309/337 (green) showing their rearrangement and fate during and after mitosis. In the time-lapse series, which can be accessed using the link provided to the Cell Centered Database, FlAsH fluorescence excitation z series along with a transmitted DIC image were recorded every 6 min for a total duration of about 5 h. 3D volume reconstructions from confocal image stacks over time are displayed at a rate of two frames per minute. From Supplemental movie 2 from Boassa et al. (2010) Traffic. PMID 20716111.

Technical Details

MDCK cells stably expressing two internal 4C domains in the C-terminus (FLNCCPGCCME)-tagged Cx43 (Cx43-4C309/337) were labeled for 1 h at 37 degrees C with 180 nM FlAsH-EDT2/12.5 uM EDT in Hanks balanced salt saline (HBSS). Free and non-specifically bound FlAsH was removed by washing with 2,3-dimercapto-1-propanol (BAL, 500 uM, 20min at 37 degrees C in HBSS). Time-lapse imaging was conducted using an Olympus FluoView1000 confocal microscope equipped with a temperature controlled chamber (at 37 degrees C) and a 60X 1.42 NA objective. Medium: Opti-MEM supplemented with 5% FBS covered with a glass coverslip.

Biological Sources
NCBI Organism Classification
Canis lupus familiaris
Cell Type
epithelial cell
Cell Line
Cellular Component
connexon complex
Biological Context
Biological Process
gap junction assembly
Molecular Function
connexin binding
Daniela Boassa
CCDB 7796
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 512px ——
X 512px ——