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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40218*  Cite 

Single computed slice through a tomographic reconstruction of the cell body of a protoplasmic astrocyte in a 0.5 um thick section from the hippocampus of a 1 month old male mouse, imaged with intermediate voltage electron microscopy. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. The reconstruction is the 1st in a series of 26 serial reconstructions through the cell soma. All 26 images can be found in this image group within the Cell Library, and the complete reconstruction can be viewed in the Cell centered database, accession # 7503.

Technical Details

Detailed methods can be found at the CCDB link provided. Briefly, one month old male mouse (C57BL/6NHsd) was perfused with Ringer's solution for 2 minutes followed by 2% glutaraldehyde and 2% paraformaldehyde in 0.15M sodium cacodylate buffer, pH 7.4. Further dissection of tissue was followed with postfixation using 1% OsO4, additional staining using 2% uranyl acetate, processed for embedding in durcupan ACM resin, and sectioned at a thickness of 5µm thick. Serial tomograms were produced from single tilt images using a JEOL4000, with 8k x 8k CCD camera, 5000X, 400.0 keV).

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
astrocyte of the hippocampus
Cellular Component
cell body
Biological Context
Biological Process
central nervous system structural organization
Mark Ellisman
Stephen Larson
Sarah Maynard
Eric Bushong
Maryann Martone
Monica Berlanga
CCDB 6856
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
osmium tetroxide
lead salt
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——