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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40199*  Cite 

Wide field mosaic of mouse hippocampus immunolabeled for the hemichannel pore protein pannexin 1 (shown here in green), the astrocytic intermediate filament GFAP (colorized blue) and the gap junction protein connexin 43 (colorized red). The section was also counterstained with DAPI (not shown). 176 tiles (16x11 frames) were digitally stitched together to generate a 3D mosaic montage. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Mouse brains were perfused with 4% paraformaldehyde, and sections at 50 µm on a vibratome. Tissue was immunostained using the Rabbit Cx43 antibody (Sigma) 1:600; Guinea pig GFAP antibody (discontinued) 1:600; and Chicken Panx1 antibody (4515) 1:250, 72hour incubation at 4C. Secondary antibodies used were donkey-anti-rabbit Al647; donkey-anti-Guinea pig-RRX; and donkey-anti-chicken-FITC, 2.5hours incubation at room temperature. Individual images were gathered using an Olympus Fluoview 1000, with an Olympus PlanApo 60X oil, 1.42 NA.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
CNS neuron (sensu Vertebrata)
astrocyte of the hippocampus
Cellular Component
intermediate filament
gap junction
Angela Cone
Gina Sosinsky
Maryann Martone
CCDB 6674
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
primary antibody plus labeled secondary antibody
Alexa Fluor 488
Alexa Fluor 647
Data Qualifiers
processed data
Sample Preparation
formaldehyde fixed tissue
glutaraldehyde fixed tissue
Relation To Intact Cell
vibratome-sectioned tissue
Spatial Axis Image Size Pixel Size
X 512px ——
Y 355px ——