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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40125*  Cite 

Confocal image of dorsal lateral striatum from a wild type mouse, immunolabeled for the vesicular monoamine transporter VMAT2 (red) and the dopamine receptor associated phosphoprotein DARRP32 (green). This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Wildtype mouse (C57BL6/129SvJ from Duke University) was perfused with aldehyde fixatives (4% paraformaldehyde + 0.1% gluteraldehyde), sectioned on Vibratome (thickness, 80 µm), and rinsed in phosphate buffered saline (PBS,3 x 10min) and incubated in blocking buffer (PBS, 3% normal donkey serum, 1% fish skin gelatin, 0.3% Triton X-100, 1% bovine serum albumin). Tissue sections incubated on shaker overnight, 4° C in primary antibodies diluted in blocking buffer as follows: anti-VMAT-2; Host = guinea pig; 1:500 (Oncogene, catalog # 503-01-50); anti-DARPP-32; Host = mouse; 1:500 (BD Transduction Laboratories, catalog #611520). Following 3 x 10' rinses, tissue was incubated for 2hr, RT, on a shaker in secondary antibodies diluted at 1:100 in working buffer (donkey-anti-mouse AF488 [Molecular Probes, Cat #A21202] and donkey-anti-guinea pig RRX [Jackson Immunoresearch Laboratories, Inc]. Following rinses in PBS, sections were mounted on slides with gelvatol. Individual images were gathered using a BioRad RTS 2000 Multiphoton with a Nikon PlanApo objective (N.A. 1.45).

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
CNS neuron (sensu Vertebrata)
Diana Price
Aki Laakso
Maryann Martone
Naoko Yamada
Andrea Thor
Monica Berlanga
Michele Cyr
CCDB 4003
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Image Type
recorded image
Image Mode
multi-photon microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of epitope
Visualization Methods
primary antibody plus labeled secondary antibody
Alexa Fluor 488
Data Qualifiers
processed data
Sample Preparation
formaldehyde fixed tissue
glutaraldehyde fixed tissue
Relation To Intact Cell
vibratome-sectioned tissue
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——