Zero degree tilt image from a tilt series collected using an intermediate voltage electron microscope through +/- 60 degrees of mitochondria isolated from mouse liver. Section thickness was 0.5 um. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database. For more information see: For more information see: Yamaguchiet al. Opa1-mediated cristae opening is Bax/Bak and BH3 dependent, required for apoptosis, and independent of Bak oligomerization. Molecular Cell 2008, 31, 557-569.
Mitochondria were isolated from mouse liver, incubated with added buffer at 37 degrees C for 12 min in AT buffer containing 10 mM KCl and centrifuged at 5200x g for 6 min, and stored in liquid nitrogen. Preparation was sectioned at a thickness of 5 µm. Single tilt tomogram images were acquired using a JEOL4000EX IVEM, 20,000X, 400.0 kV accelerating voltage, from -60 to 60° at 2° intervals.