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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40074*  Cite 
Description

Projection through merged optical section series of a filled astrocyte (green) in the molecular layer of the dentate gyrus of a 1 month-old rat, stained for NCAM (red), showing the relationship between astrocyte processes and laminar boundaries revealed by NCAM staining. Optical sections were generated with a single photon confocal microscope. Original data contributed to Bushong EA, Martone ME, Ellisman MH. Examination of the relationship between astrocyte morphology and laminar boundaries in the molecular layer of adult dentate gyrus. J Comp Neurol. 2003 Jul 21;462(2):241-51. PMID: 12794746. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Technical Details

Projection through merged optical section series of a filled astrocyte (green) in the molecular layer of the dentate gyrus of a 1 month-old rat, stained for NCAM (red), showing the relationship between astrocyte processes and laminar boundaries revealed by NCAM staining. Optical sections were generated with a single photon confocal microscope. Original data contributed to Bushong EA, Martone ME, Ellisman MH. Examination of the relationship between astrocyte morphology and laminar boundaries in the molecular layer of adult dentate gyrus. J Comp Neurol. 2003 Jul 21;462(2):241-51. PMID: 12794746. This image has been downsampled from the raw data image which can be accessed using the link provided to the Cell Centered Database.

Biological Sources
NCBI Organism Classification
Rattus norvegicus
Cell Type
astrocyte
astrocyte of the hippocampus
Cellular Component
cell projection cytoplasm
cell body
Biological Context
Biological Process
forebrain astrocyte development
Attribution
Names
Eric A. Bushong
Maryann E. Martone
Mark H. Ellisman
Pubmed
PMID:12794746
Link
CCDB 3775
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40074
Archival Resource Key (ARK)
ark:/b7295/w9cil40074
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
intracellular filling
distribution of epitope
Visualization Methods
Alexa Fluor 568
primary antibody plus labeled secondary antibody
Alexa Fluor 488
Data Qualifiers
processed data
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
vibratome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——