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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:40026*  Cite 
Description

A maximum intensity projection of a Caulobacter crescentus specimen at 0 degree tilt.

Technical Details

Caulobacteria were induced for 1 hour at 30 degrees C, labeled in M2G medium for 2 hours at 30 degrees C (in the presence of 1.8 micromolar ReAsH-EDT2) and photoconverted. Samples were fixed with 2.5% glutaraldehyde with 1% acrolein in 0.05M sodium cacodylate pH 7.4 with 3 mM CaCl2 and 1 % sucrose followed by conventional osmium tetroxide/potassium ferrocyanide post-fixation, dehydration and embedding, and sectioning at 4µm thickness. A single tilt series of images were acquired using a JEOL 4000, from -60 to 60° at 2° increments, magnification, 40000.0.

Biological Sources
NCBI Organism Classification
Caulobacter crescentus CB15
Cell Type
prokaryotic cell
Attribution
Names
Lucy Shapiro
Harley McAdams
Link
CCDB 3700
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL40026
Archival Resource Key (ARK)
ark:/b7295/w9cil40026
Imaging
Image Type
recorded image
Image Mode
Parameters Imaged
electron density
Data Qualifiers
processed data
Sample Preparation
Methods
acrolein fixed tissue
glutaraldehyde fixed tissue
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 512px ——
X 512px ——